Long-acting hormone and growth factor compositions and uses thereof

ABSTRACT

This invention provides VEGF-FSH compounds having increased serum half-lives relative to either native VEGF or FSH, in which both VEGF and FSH are biologically active. This invention also provides related compositions and methods for increasing fertility, egg production and spermatogenesis in a subject, as well as methods for increasing vascularization in a tissue, particularly in ovarian tissue.

[0001] This application is a continuation-in-part of U.S. Ser. No. 10/119,427, filed Apr. 9, 2002, and claims priority of U.S. Ser. No. 10/062,931, filed Jan. 31, 2002, now abandoned, the contents of which are hereby incorporated into this application by reference.

[0002] Throughout this application, various publications are referenced by author and publication date. Full citations for these publications may be found at the end of the specification immediately preceding the claims. The disclosure of these publications is hereby incorporated by reference into this application to describe more fully the art to which this invention pertains.

[0003] The invention described herein was made with government support under grant number DK-51266 from the National Institutes of Health. Accordingly, the United States government has certain rights in this invention.

BACKGROUND OF THE INVENTION

[0004] One impediment to gonadotropin hormone replacement therapy has been the need for frequent repeated injections in order to maintain efficacious serum levels of the hormone. For example, current pharmacological formulations of follicle stimulating hormone (FSH) must be administered daily, often for 8 to 12 days when used to induce ovulation (LeContonnec et al, 1994). This dosing regimen is required due to the relatively short serum half-life of FSH. Thus, the development of more stable, longer-acting FSH formulations Glycoprotein hormones, including FSH, luteinizing hormone (LH), thyrotropin stimulating hormone (TSH) and chorionic gonadotropin (CG), are heterodimers comprised of two non-covalently bound subunits, α and β (Pierce et al, 1981). While the α-subunit is interchangeable among the hormones of this family, the β-subunit is unique and primarily responsible for the biological specificity of hormone action (see FIGS. 17 and 18 for the sequences of the hFSH β and α subunits, respectively).

[0005] The β subunits of hCG and hLH share greater than 85% sequence identity through the N-terminal 112 amino acids (Pierce et al, 1981). In addition, these two hormones share a common receptor and elicit similar biological activity following receptor binding. However, the serum half-life of hCG is almost five times that of hLH (Porchet et al, 1995; Saal et al, 1991; Yen et al, 1968). The main structural difference between β-hCG and β-hLH is an additional carboxy terminal peptide sequence on the β-hCG subunit, corresponding to amino acids 113-145 (Matzuk et al, 1990). This carboxy-terminal peptide, or “CTP”, is now known to be the key determinant of the decreased metabolism and excretion of hCG, and thus of its long serum half-life, the longest among the glycoprotein hormones. The biological role of the carboxy-terminal extension of hCG is discussed in Matzuk et al, (1990). Surprisingly, the sequence of the CTP peptide itself is not responsible for the stability of this protein. Rather, it is the presence of four O-linked sugars that confer its remarkable stability. The characterization of the O-glycosylation sites of the hCG β subunit is described in Sugahara et al, (1996).

[0006] Many integral membrane proteins and secretory proteins are glycosylated. The most common type of glycosylation, called “N-linked”, occurs when a sugar moiety is linked to the amide nitrogen of an asparagine residue within the consensus sequence Asn-X-Ser or Asn-X-Thr. The carbohydrate moieties of the gonadotropin hormones play an important role in the hormone's functionality. For example, appropriate glycosylation is required for protein folding and hormonal signal transduction. The importance of the asparagine-linked oligosaccharides of the hCG β subunit in correct disulfide bond pairing and thus on assembly and secretion are discussed in Feng et al, (1995) and Matzuk et al, (1990).

[0007] The O-linked glycosylations of the CTP sequence of hCG are so-named because the sugar moiety is linked to the hydroxyl group of either serine or threonine. This kind of linkage is much less common and, in contrast to N-linked moieties, O-linked sugars are not required for the hormonal activity of hCG. Schematic examples of N-linked and O-linked carbohydrates are shown in FIG. 14.

[0008] The importance of the CTP in promoting hormone stability was demonstrated by the construction of a fusion protein consisting of the CTP portion of the β-subunit of hCG and the carboxy terminus of β-hFSH (Fares et al, 1992). This β-hFSH-CTP fusion produced a long-acting hFSH agonist which was able to dimerize with a coexpressed α-subunit to produce a functional FSH hormone. Importantly, this β-hFSH-CTP demonstrated similar in vitro bioactivity and substantially increased in viva bioactivity compared with preparations of native hFSH.

[0009] Thus, merely adding the CTP sequence to β-hFSH was sufficient to increase the biological activity of the hormone, most likely through an increase in serum-half life. Indeed, recent pharmacokinetic parameter estimates in humans have demonstrated that this β-hFSH-CTP analog has an elimination half-life 2 to 3 times longer than that of native recombinant hFSH (Bouloux et al, 2001).

[0010] While the administration of pharmacological preparations of hFSH alone has been used in the treatment of infertility, this approach is likely to be enhanced substantially by methodologies which simultaneously induce follicular vascularization.

[0011] The primary mediator of follicular vascularization is VEGF, which is induced in response to FSH stimulation (Dissen et al, 1994). Reproductive aging is associated with diminished follicular vascularity, resulting somewhat paradoxically in an increase of VEGF, presumably in response to local hypoxia (van Blerkom et al, 1997). However, this adaptive response is often inadequate to reestablish appropriate vascular supply to the area.

[0012] Notably, improved vascularization has clinically been associated with improved oocyte yield, embryo morphology and pregnancy rates (Chui et al, 1997; Nargund et al, 1996). This is because perifollicular blood vessel development is critical to the health and integrity of the growing follicle.

[0013] Pharmacologic doses of exogenous VEGF can significantly ameliorate perifollicular hypoxia and improve follicular development in some patients. However, attempts to provide exogenous VEGF have suffered from severe side effects associated with the systemic administration of this growth factor.

SUMMARY OF THE INVENTION

[0014] This invention provides a first composition of matter comprising at least one subunit of a hormone or growth factor and a half-life increasing moiety, wherein the hormone or growth factor subunit and the half-life increasing moiety are covalently bound.

[0015] This invention also provides a second composition of matter comprising at least one subunit of a hormone or growth factor, a half-life increasing moiety, and a moiety that specifically binds to cells of a predetermined tissue.

[0016] This invention also provides a third composition of matter comprising at least one subunit of a hormone or growth factor, a β-FSH subunit, an α-FSH subunit and a half-life increasing moiety, wherein the hormone or growth factor, the β-FSH subunit, the α-FSH subunit and the half-life increasing moiety are covalently bound.

[0017] This invention also provides a pharmaceutical composition comprising any one of the instant compositions of matter and a pharmaceutically acceptable carrier.

[0018] This invention provides nucleic acids encoding the instant synthetic polypeptides, as well as expression vectors and suitable host cells for expressing same.

[0019] This invention also provides a method for producing the polypeptides of the instant invention that comprises growing a suitable host cell transfected with a vector encoding the polypeptide under conditions permitting its expression and recovering the polypeptide so expressed.

[0020] This invention also provides a method for increasing a subject's fertility which comprises administering to the subject an amount of the third instant composition effective to enhance the subject's fertility.

[0021] This invention also provides a method for increasing a subject's egg production which comprises administering to the subject an amount of the third instant composition effective to enhance the subject's egg production.

[0022] This invention also provides a method for increasing spermatogenesis in a subject through administering to the subject an amount of the third instant composition effective to enhance the subject's spermatogenesis.

[0023] This invention also provides a method for increasing vascularization in a tissue by contacting the tissue with an amount of a composition of the instant invention effective to increase vascularization in the tissue.

[0024] Finally, this invention provides a method for increasing the vascularization in ovarian tissue comprising contacting the tissue with an amount of the first instant composition, comprising VEGF, effective to increase vascularization in the ovarian tissue.

BRIEF DESCRIPTION OF THE FIGURES

[0025]FIG. 1: Nucleotide and predicted amino acid sequence of the fusion protein β-hFSH-N2. The N2 sequence contains amino acids 130 through 146.

[0026]FIG. 2: Nucleotide and predicted amino acid sequence of the fusion protein β-hFSH-N4. The N4 sequence contains amino acids 130 through 161.

[0027]FIG. 3: Nucleotide and predicted amino acid sequence of the fusion protein β-hFSH-CTP-α-hFSH.

[0028]FIG. 4: Nucleotide and predicted amino acid sequence of the fusion protein β-hFSH-N2-α-hFSH.

[0029]FIG. 5: Nucleotide and predicted amino acid sequence of the fusion protein β-hFSH-N4-α-hFSH.

[0030]FIG. 6: (Panel A) Schematic β-hFSH-CTP-α construct; (Panel B) Schematic β-hFSH-N2/N4-α construct; (Panel C) Schematic VEGF-FSH-CTP construct; (Panel D) Schematic VEGF-FSH-N2/N4 construct.

[0031]FIG. 7: Schematic of β-hFSH-CTP-α-hFSH construct with locations of restriction sites.

[0032]FIG. 8: In vitro bioassay of hormone activity. cAMP concentration (fmol/ml) was quantitated in Y1 cells expressing the FSH receptor after treatment with the indicated amount of either pituitary FSH (pFSH) or the FSH analogue, β-hFSH-CTP-α-hFSH (rFSH-CTP).

[0033]FIG. 9: Serum levels of the FSH analogue, β-hFSH-CTP-α-hFSH (hFSH-CTP), in 4 rhesus monkeys (indicated by triangles, inverted triangles, circles, and squares, respectively) measured at the indicated times following a single subcutaneous injection at a dose of 10 IU/kg.

[0034]FIG. 10: Serum levels of the control recombinant hFSH protein, (hFSH), in 2 rhesus monkeys (indicated by diamonds and triangles, respectively) measured at the indicated times following a single subcutaneous injection at a dose of 10 IU/kg.

[0035]FIG. 11: Serum estradiol levels in two normally cycling monkeys following a single subcutaneous injection of the FSH analogue, β-hFSH-CTP-α-hFSH (FSH-CTP). The time of injection is indicated by arrows. Both monkeys were given the GnRH antagonist Ganirelix Acetate for the duration of the study.

[0036]FIG. 12: Amino acid sequence of β-hCG, wherein CHO is a glycosylation site and the black shading corresponds to the CTP. N-linked glycosylation is present on Asn, and O-linked glycosylation is present on Ser.

[0037]FIG. 13: Schematic of the carbohydrate moieties on both hLH and hFSH and some of the microheterogeneity which results in the wide range of isoelectric points in the glycoprotein hormones.

[0038]FIG. 14: Schematic examples of N-linked and O-linked carbohydrates.

[0039]FIG. 15: Nucleotide and amino acid sequence of β-hFSH. The signal sequence corresponds to the sequence beginning with the methionine at position 1 and ending with the cysteine at position 18.

[0040]FIG. 16: Nucleotide and amino acid sequence of α-hFSH. The signal sequence corresponds to the sequence beginning with the methionine at position 1 and ending with the serine at position 24.

[0041]FIG. 17: Mean ovarian weight three days following subcutaneous injection of either recombinant human FSH (rFSH), FSH-CTP, N-2, N4, or saline.

[0042]FIG. 18: Mean serum hFSH concentration-time profiles following a single IV injection of either recombinant human FSH (rFSH, bottom), FSH-CTP (middle) or N-2 (top). Twenty-one day old female rats were injected at a dose of 2800 ng/rat.

[0043]FIG. 19: In vitro bioactivity of VEGF-FSH (inverted triangle), recombinant native hFSH (circle and diamond), synthetic FSH-CTP (triangle).

[0044]FIG. 20: Effect of VEGF-FSH on endothelial cell proliferation as determined by MTT assay. Panel A, native VEGF or VEGF-FSH was added to the medium at the indicated concentration. Panel B, cells were incubated with either EBM media, or a 1:1 mixture of EBM media and CHO cell supernatant taken from cells secreting either synthetic FSH-CTP or VEGF-FSH, as indicated.

[0045]FIG. 21: Phosphorylation of VEGF receptor. Cells were incubated with either native VEGF, VEGF-FSH (VFC), or synthetic FSH-CTP (hFSH-CTP), as indicated by arrows.

[0046]FIG. 22: Ovarian H&E sections taken from mice injected with either saline (Panel A), synthetic FSH-CTP (Panel B), VEGF-FSH (Panel C), or a mixture of FSH-CTP and native VEGF (Panel D).

[0047]FIG. 23: Representative ovarian H&E section showing perifollicular capillaries (arrows).

DETAILED DESCRIPTION OF THE INVENTION Definitions

[0048] The terms “amino acid,” “amino acid residue” or “residue” are used interchangeably herein to refer to an amino acid that is incorporated into a protein, polypeptide or peptide. The amino acid can be, for example, a naturally occurring amino acid or an analog of a natural amino acid that can function in a similar manner as the naturally occurring amino acid.

[0049] As used herein, “CTP” means the carboxy-terminal peptide of β-hCG, corresponding to amino acid residues 113-145. This portion of hCG contains multiple O-linked glycosylation sites (see FIG. 12).

[0050] The letter “h” is used herein to designate the human isoform of a protein or polypeptide. For example, hFSH means human follicle stimulating hormone. FSH is a pituitary glycoprotein essential for follicular growth as well as spermatogenesis, comprised of a noncovalently linked heterodimer of two peptide subunits, α and β. The β subunit is specific to FSH and thus determines its biological activity, while the α subunit is common to the other members of this glycoprotein family, for example, luteinizing hormone (LH), chorionic gonadotrophin (CG) and thyroid-stimulating hormone (TSH).

[0051] The terms “nucleic acid”, “polynucleotide” and “nucleic acid sequence” are used interchangeably herein, and each refers to a polymer of deoxyribonucleotides and/or ribonucleotides. The deoxyribonucleotides and ribonucleotides can be naturally occurring or synthetic analogues thereof.

[0052] The terms “polypeptide,” “peptide” and “protein” are used interchangeably herein, and each means a polymer of amino acid residues. The amino acid residues can be naturally occurring or chemical analogues thereof. Polypeptides, peptides and proteins can also include modifications such as glycosylation, lipid attachment, sulfation, hydroxylation, and ADP-ribosylation.

[0053] As used herein, “serum half-life”, abbreviated “t_(1/2)”, means elimination half-life, i.e., the time at which the serum concentration of an agent has reached one-half its initial or maximum value. The term “increased serum half-life” used herein in reference to a synthetic agent means that the synthetic agent is cleared at a slower rate than either the non-synthetic, endogenous agent or the recombinantly produced version thereof. For example, the t_(1/2) of a synthetic FSH, e.g., hFSH-N2, in a subject would be “increased” if it exceeds the t_(1/2) of either endogenous FSH or recombinantly produced native FSH.

[0054] As used herein, a “signal sequence” is an N-terminal portion of the nascent polypeptide chain that directs the polypeptide to the export apparatus of the cell and is itself cleaved from the nascent polypeptide, in general before postranslational modification and secretion of the mature protein. The signal sequences and associated DNA region encoding these peptides for the beta and alpha hFSH chains are shown in FIGS. 15 and 16, respectively.

[0055] As used herein, “suitable host cells” include, but are not limited to, bacterial cells, yeast cells, fungal cells, insect cells, and mammalian cells. Mammalian cells can be transfected by methods well-known in the art such as calcium phosphate precipitation, electroporation and microinjection.

[0056] As used herein, “vector” means any nucleic acid vector known in the art. Such vectors include, but are not limited to, plasmid vectors, cosmid vectors, and bacteriophage vectors.

[0057] Units, prefixes and symbols may be denoted in their SI accepted form. Unless otherwise indicated, nucleic acid sequences are written left to right in 5′ to 3′ orientation and amino acid sequences are written left to right in amino- to carboxy-terminal orientation. Amino acids may be referred to herein by either their commonly known three letter symbols or by the one-letter symbols recommended by the IUPAC-IUB Biochemical Nomenclature Commission. Nucleotides, likewise, may be referred to by their commonly accepted single-letter codes.

[0058] In this invention, administering the instant pharmaceutical composition can be effected or performed using any of the various methods and delivery systems known to those skilled in the art. The administering can be performed, for example, intravenously, orally, via implant, transmucosally, transdermally, intramuscularly, and subcutaneously. In addition, the instant pharmaceutical compositions ideally contain one or more routinely used pharmaceutically acceptable carriers. Such carriers are well known to those skilled in the art. The following delivery systems, which employ a number of routinely used carriers, are only representative of the many embodiments envisioned for administering the instant composition.

[0059] Injectable drug delivery systems include solutions, suspensions, gels, microspheres and polymeric injectables, and can comprise excipients such as solubility-altering agents (e.g., ethanol, propylene glycol and sucrose) and polymers (e.g., polycaprylactones and PLGA's). Implantable systems include rods and discs, and can contain excipients such as PLGA and polycaprylactone.

[0060] Oral delivery systems include tablets and capsules. These can contain excipients such as binders (e.g., hydroxypropylmethylcellulose, polyvinyl pyrilodone, other cellulosic materials and starch), diluents (e.g., lactose and other sugars, starch, dicalcium phosphate and cellulosic materials), disintegrating agents (e.g., starch polymers and cellulosic materials) and lubricating agents (e.g., stearates and talc).

[0061] Transmucosal delivery systems include patches, tablets, suppositories, pessaries, gels and creams, and can contain excipients such as solubilizers and enhancers (e.g., propylene glycol, bile salts and amino acids), and other vehicles (e.g., polyethylene glycol, fatty acid esters and derivatives, and hydrophilic polymers such as hydroxypropylmethylcellulose and hyaluronic acid).

[0062] Dermal delivery systems include, for example, aqueous and nonaqueous gels, creams, multiple emulsions, microemulsions, liposomes, ointments, aqueous and nonaqueous solutions, lotions, aerosols, hydrocarbon bases and powders, and can contain excipients such as solubilizers, permeation enhancers (e.g., fatty acids, fatty acid esters, fatty alcohols and amino acids), and hydrophilic polymers (e.g., polycarbophil and polyvinylpyrolidone).

[0063] Solutions, suspensions and powders for reconstitutable delivery systems include vehicles such as suspending agents (e.g., gums, zanthans, cellulosics and sugars), humectants (e.g., sorbitol), solubilizers (e.g., ethanol, water, PEG and propylene glycol), surfactants (e.g., sodium lauryl sulfate, Spans, Tweens, and cetyl pyridine), preservatives and antioxidants (e.g., parabens, vitamins E and C, and ascorbic acid), anti-caking agents, coating agents, and chelating agents (e.g., EDTA).

Embodiments of the Invention

[0064] This invention provides a composition of matter comprising at least one subunit of a hormone or growth factor and a half-life increasing moiety, wherein the hormone or growth factor subunit and the half-life increasing moiety are covalently bound.

[0065] Half-life increasing moieties include, for example, a peptide containing one or more glycosylation sites. A half-life increasing moiety can also be nonpeptidyl, either in whole or in part, for example, polyethylene glycol.

[0066] In certain embodiments of the instant compositions of matter, the glycosylation is either O-linked or N-linked glycosylation. The number of glycosylation sites may be any number, such as one, two, three, four, five, or six sites. In a preferred embodiment, each site is separated from its adjacent site by about six amino acid residues.

[0067] In a preferred embodiment of the instant invention, the hormone or growth factor subunit and the polypeptide segment exist within a single polypeptide chain.

[0068] In an embodiment of any of the instant compositions of matter, the hormone or growth factor is selected from the group consisting of VEGF, GH, TGFα, TGFβ1, EGF, FGF, SCF, IGF-I, IGF-II, GDF-9, KGF, BMP-15, GM-CSF, LIF, follistatin, activin-β, a neurotropin, cadherin, angiopoiten I or II, MCP-1, ICAM-1, CD18, P-selectin and MIP-2. In a preferred embodiment, the growth factor is VEGF.

[0069] In an embodiment of any of the instant compositions of matter, the hormone or growth factor is from an animal selected from the group consisting of a primate, a horse, a sheep, a bird, a bovine, a pig, a dog, a cat and a rodent. In a preferred embodiment, the hormone or growth factor subunit is from a human.

[0070] In one embodiment of the first composition, the hormone or growth factor subunit is bound at its C-terminal end to the N-terminal end of the polypeptide segment. In another embodiment, the hormone or growth factor is bound at its N-terminal end to the C-terminal end of the polypeptide segment.

[0071] In yet a further embodiment of the first composition, the polypeptide segment comprises the carboxy-terminal portion of the β-hCG subunit. In the preferred embodiment, the carboxy-terminal subunit comprises the amino acid sequence corresponding to positions 113-145 of the β-hCG subunit.

[0072] The carboxy-terminal portion of the β-hCG subunit is preferably glycosylated on one or more serine residues, constituting one or more O-linked glycosylation sites. This polypeptide segment can also comprise a region having one or more N-linked glycosylation sites.

[0073] As used herein, an “N-linked” glycosylation site includes, without limitation, asn followed by any of X-ser, X-thr and X-cys, wherein X is any amino acid except proline, and glycosylation occurs on the asn residue. In this invention, the amino acid sequence of any polypeptide situated N-terminal to, C-terminal to, or in between two N-linked sites, can be of any content and length needed to suit a particular design requirement.

[0074] In another embodiment of the first composition, the polypeptide segment comprises the amino acid sequence ser-gly-ser-asn-ala-thr-gly-ser-gly-ser-asn-ala-thr-ser-gly-ser.

[0075] In an embodiment of any of the instant compositions of matter, wherein the half-life increasing moiety is a polypeptide segment having the amino acid sequence ser-gly-ser-asn-ala-thr-gly-ser-gly-ser-asn-ala-thr-ser-gly-ser, the polypeptide segment comprises one or a plurality of the amino acid sequence.

[0076] This invention also provides a second composition of matter comprising at least one subunit of a hormone or growth factor, a half-life increasing moiety, and a moiety that specifically binds to cells of a predetermined tissue.

[0077] This invention further provides a novel fusion protein incorporating VEGF, FSH and a half-life increasing moiety. The FSH moiety serves both to target the fusion protein to the ovaries and as a stimulus to follicular maturation. Importantly, both the VEGF and FSH thus targeted to the ovaries are biologically active as demonstrated by increased follicular vascularization and maturation. Thus, the present invention provides a significant development in the field of gonadotropin hormone replacement therapy.

[0078] Specifically, this invention provides a third composition of matter comprising at least one subunit of a hormone or growth factor, a β-FSH subunit, an α-FSH subunit and a half-life increasing moiety, wherein the hormone or growth factor, the β-FSH subunit, the α-FSH subunit and the half-life increasing moiety are covalently bound.

[0079] In one embodiment of the third composition, the growth factor is VEGF. In a further embodiment the α-FSH and β-FSH subunits are from an animal selected from the group consisting of a primate, a horse, a sheep, a bird, a bovine, a pig, a dog, a cat and a rodent.

[0080] In a preferred embodiment of the third composition, the α-FSH and β-FSH subunits are human α-FSH and β-FSH subunits which exist in a single chain polypeptide along with the VEGF and the half-life increasing moiety.

[0081] In a further embodiment of the third composition, the β-FSH subunit is bound at its C-terminal end to the N-terminal end of the polypeptide segment, and the polypeptide segment is bound at its C-terminal end to the N-terminal end of the α-FSH subunit. In this embodiment, the synthetic FSH comprises the N-terminal signal sequence of the β-FSH subunit.

[0082] In another embodiment of the third composition, the α-FSH subunit is bound at its C-terminal end to the N-terminal end of the polypeptide segment, and the polypeptide segment is bound at its C-terminal end to the N-terminal end of the β-FSH subunit.

[0083] In yet another embodiment of the third composition, the VEGF is bound at its C-terminal end to the β-FSH subunit, the β-FSH is bound at its C-terminal end to the N-terminal end of the polypeptide segment, and the C-terminal end of the polypeptide segment is bound to the N-terminal end of the α-FSH subunit.

[0084] This invention also provides a pharmaceutical composition comprising any one of the instant compositions of matter and a pharmaceutically acceptable carrier.

[0085] This invention provides nucleic acids encoding the instant synthetic molecules, as well as expression vectors and suitable host cells for expressing -said molecules. Examples of vectors include a plasmid, a cosmid, λ phage and a yeast artificial chromosome, abbreviated “YAC”. Any suitable cell system may be used to express the synthetic FSH molecules of the instant invention. For example, synthetic FSHs of the instant invention may be expressed in a bacterial cell or in a eukaryotic cell. In a preferred embodiment, a synthetic FSH is expressed in a Chinese hamster ovary cell, since this cell type provides certain advantageous post-translational protein modifications.

[0086] This invention also provides a method for producing a polypeptide that comprises growing a cell, for example a Chinese hamster ovary cell, under conditions permitting expression of the polypeptide encoded by the vector therein, and recovering the polypeptide so expressed. In a preferred embodiment, the vector encoding the polypeptide is transfected into the cells and subcultured under conditions that favor the growth of those cells which have taken up the vector. For example, the vector may contain one or more antibiotic resistance genes. Thus, medium containing the antibiotic will favor the growth of only those cells which have been transfected with the vector.

[0087] In a preferred embodiment, the polypeptide contains a signal sequence that targets the polypeptide for excretion from the cell. In a further embodiment, the excreted polypeptide may be collected, purified, and concentrated, for example by affinity chromatography, gel electrophoresis, and vacuum-assisted evaporation.

[0088] This invention also provides a method for increasing a subject's fertility which comprises administering to the subject an amount the third instant composition effective to enhance the subject's fertility. Determining a therapeutically effective amount of the instant composition can be done based on animal data using routine computational methods.

[0089] In one embodiment, this method is used to enhance the efficiency of in vitro fertilization protocols. For example, a composition of matter of the instant invention can enhance the success of in vitro fertilization by stimulating follicular maturation and egg production in the subject.

[0090] In a preferred embodiment of the instant invention, the composition is administered to the subject less frequently than current methods allow. For example, the instant composition may be administered every other day, every 6 to 8 days, or weekly. The instant composition can also be administered daily.

[0091] This invention also provides a method for increasing a subject's egg production which comprises administering to the subject an amount of the third instant composition effective to enhance the subject's egg production.

[0092] This invention further provides a method for increasing spermatogenesis in a subject through administering to the subject an amount of the third instant composition effective to enhance the subject's spermatogenesis.

[0093] This invention also provides a method for increasing vascularization in a tissue by contacting the tissue with an amount of a composition of the instant invention effective to increase vascularization in the tissue. The tissue may be, for example, heart, lung, vascular, testicular or dermal tissue. In a preferred embodiment, the tissue is ovarian tissue.

[0094] As used herein, a subject can be, for example, a primate, a horse, a sheep, a bird, a bovine, a pig, a dog, a cat, or a rodent. In the preferred embodiment, the subject is a human.

[0095] Finally, this invention provides a method for increasing the vascularization in ovarian tissue comprising contacting the tissue with an amount of the first instant composition, comprising VEGF, effective to increase vascularization in the ovarian tissue.

[0096] This invention is illustrated in the Experimental Details section which follows. This section is set forth to aid in an understanding of the invention but is not intended to, and should not be construed to, limit in any way the invention as set forth in the claims which follow thereafter.

EXPERIMENTAL DETAILS Methods

[0097] General

[0098] Cloning and preparation of plasmid DNA were performed with E. coli strain DH5α. Clones were grown in standard Luria-Bertani medium (LB) for purification of recombinant DNA constructs. Transformation of DH5α was performed according to standard techniques using calcium chloride.

[0099] PCR reactions were performed with Vent DNA polymerase (New England Biolabs, Beverly, Mass.) and all products of the reactions were sequenced to ensure that no mutations were introduced during the amplification.

[0100] Construction of the β-hFSH-CTP-α-hFSH Fusion Protein

[0101] An in-frame Xho I site was inserted adjacent to the initation codon of the β-hFSH cDNA sequence and a Bam HI site was inserted adjacent to the final codon, eliminating the terminator codon. Similarly, a Bam HI site was inserted adjacent to the codon for residue 113 in the cDNA encoding CTP and an Xba I site was inserted adjacent to the codon for residue 145. These two fragments were then ligated to form a contiguous Xho I-Bam HI-Xba I β-hFSH-CTP fusion lacking a terminator codon. This fusion was then ligated with a cDNA encoding the mature α-subunit which lacked the amino-terminal signal peptide but included the terminator codon, flanked by in-frame 5′ Spe I and 3′ Sac I sites. The final construct encodes a fusion of the β-hFSH and α-subunit with the CTP sequence as the linker sequence. This final fusion sequence was then inserted into an SV40 expression vector.

[0102] Construction of the β-hFSH-N2/N4-α-hFSH Fusion Protein

[0103] The β-hFSH-N2 and -N4 constructs consist of a single polypeptide chain hFSH molecule containing the β- and α-subunits tethered by a synthetic polypeptide consisting of either one or two tandem copies of the following: Ser-Gly-Ser-Asn-Ala-Thr-Gly-Ser-Gly-Ser-Asn-Ala-Thr-Ser-Gly-Ser. β-hFSH-N2 was constructed by synthesizing two complementary DNA strands encoding the above polypeptide in one of six potential reading frames. These two DNAs were designed such that following annealing a 5′ Bam HI end and a 3′ Spe I end were formed. The synthetic DNA duplex was then ligated into a vector with the hFSH β- and α-subunit encoding cDNAs. The in-frame ligation of these three DNAs was accomplished by placing a Xho I site immediately preceding the start codon and replacing the terminator codon of the hFSH β-subunit with a Bam HI site. In addition, an Spe I site was placed at the 5′ end and a Sac I site immediately following the terminator codon of the α-subunit. The three fragments were then inserted into an SV40-based expression vector at Xho I/Sac I sites to form the β-hFSH-N2 expression construct. To insert a second copy of the synthetic polypeptide, a Bgl II site was inserted at the end of the synthetic sequence in the β-hFSH-N2 clone immediately preceding the Spe I site. The second copy of the synthetic polypeptide was then inserted by cleaving the β-hFSH-N2 construct with Bgl II and Spe I followed by insertion of the Bam HI/Spe I ended synthetic DNA to form β-hFSH-N4. This was feasible since Bam HI and Bgl II have identical cohesive termini.

[0104] Construction a VEGF-β-hFSH-CTP-α-hFSH (VEGF-FSH-CTP) Fusion

[0105] A 165-residue isoform of VEGF-A containing the heparin-binding domain was used herein. A cDNA encoding this isoform and its signal sequence but lacking the terminator codon was produced with an Xba I site at the 3′ end. This CDNA was directly linked to an Xba I site placed at the 5′ end of a single chain hFSH clone lacking the start codon and signal sequence, thereby fusing VEGF¹⁶⁵ to β-hFSH-CTP. The signal peptide on the VEGF¹⁶⁵ moiety was left unchanged for secreting the fused chimera. This particular orientation was chosen based on both crystallographic and growth factor activity analyses which suggested that the carboxy terminus of VEGF was more flexible and less directly involved in functional activities.

[0106] Construction a Long-Acting VEGF Protein (VEGF-N2)

[0107] A long-acting VEGF protein was synthesized by tethering the VEGF¹⁶⁵ protein described above to a peptide having two tandem copies of the N-linked glycosylation signal sequence. The resulting fusion protein is designated VEGF-N2. As described above, the VEGF-N2 construct was transfected into CHO lines for protein production and purification. The synthetic protein was detected by immunoblotting with anti-VEGF antibodies.

[0108] Expression of Fusion Proteins

[0109] An SV40 expression clone containing the fusion construct was co-transfected into Chinese hamster ovary cells (CHO-K1) along with an SV2neo clone encoding resistance to the antibiotic G418. The CHO cell transformation was performed using a standard calcium phosphate precipitate technique. Media containing G418 (Gemini Bioproducts, Woodland, Calif.) was used to select transfected cells and media was assayed for secretion of the fusion protein using an hFSH-specific radioimmunoassay. Isolated colonies were pooled and maintained in Ham's F-12 culture medium containing 500 ug/mL G418, 10% fetal bovine serum, 100 units/mL penicillin, 100 ug/mL streptomycin, and 4 mM glutamine. Pooled colonies were subcloned in 96 well microtiter dishes and high-secreting clones were isolated. To further increase the yield of the secreted fusion proteins, the cells were grown in suspension cultures.

[0110] Purification of Fusion Proteins

[0111] Spinner bottles were seeded at 10⁵ cells/mL in CHO-S-SFM medium (Life technologies, Rockville, Md.) containing 400 ug/mL G418. Cultures generally reached a density of 2×10⁶ cells/mL on day 6 or 7, and the cell supernatant was harvested on day 7 or 8. PMSF was added to the supernatant at a concentration of 0.2 mM, which was then filtered through a 0.2 μm membrane and stored at 4° C. Affinity purification of was accomplished using an A201 (α-subunit specific antibody column). The column was prepared by coupling purified A201 immunoglobulins to CNBr-Sepharose-4B according to the manufacturer's instructions (Amersham Pharmacia Biotech, Piscataway, N.J.) at a concentration of 5 mg antibody/mL Sepharose. After applying the cell supernatant, the column was washed with 50 bed volumes of PBS followed by 2 bed volumes of distilled water. The fusion protein was eluted with 3-4 bed volumes of 1 M acetic acid and immediately dried on a Speed-Vac concentrator (Savant Instruments, Holbrook, N.Y.).

[0112] Subcutaneous Protocol

[0113] Rhesus monkeys were injected subcutaneously with the fusion protein (n=4) or r-hFSH (Follistim, Organon Inc., n=2) at a dose of 10 IU/kg. All except 1 of the monkeys in each of the two treatment groups had been ovariectomized prior to injection. Serum hFSH was assayed prior to injection and at the following intervals post-injection: 12 h, 16 h, 20 h, 24 h, 36 h, 48 h, 60 h, and every 24 hours thereafter until levels reached baseline (approximately 9 days for control animals, 19-22 days for treatment animals).

[0114] Intravenous (IV) Protocol

[0115] One rhesus monkey was given an IV bolus of the fusion protein (10 IU/kg). A second animal was given an IV bolus of the control, r-hFSH at the same dose. Serum was assayed for hFSH prior to bolus administration and at the following intervals post-injection: 0.5 h, 1 h, 2 h, 4 h, 8 h, 12 h, 24 h, 36 h, 48 h, 72 h, 96 h, 120 h, 144 h and 168 h.

[0116] Pharmacokinetics

[0117] Recombinant human FSH (r-hFSH) (Follistim, Organon Inc, West Orange, N.J.) was used as a control. The Immulite assay (Diagnostic Products Corporation, Los Angeles, Calif.) was used to quantitate hFSH protein. This assay was able to detect the hFSH analogues in vitro and in vivo, and did not cross-react with rhesus FSH.

[0118] Pharmacokinetic Analysis

[0119] Each individual data set was evaluated by the pharmacokinetic data analysis program PKAnalyst (Micromath, Inc., Salt Lake City, Utah). For the IV dosing study, the following biexponential equation was fitted to the data: C(t)=Ae-at+Be-bt, where C(t) is the plasma concentration at time “t”, and A and B are the multiexponential coefficients. Values of a and b represent the initial-phase disposition rate constant and the terminal-phase disposition rate constant, respectively. PKAnalyst was used to generate the best-fit critical pharmacokinetic parameters, including elimination rate constant, half-life of initial (distribution) phase (t_(1/2a)), half-life of terminal (elimination) phase (t_(1/2b)), and total area under the blood concentration-time curve (AUC).

[0120] For the subcutaneous dosing studies, the blood concentration-time data were represented by the following biexponential equation: C(t)=A(e-Ket−e-Kat), where C(t) is the blood concentration at time “t” and A the multiexponential coefficient. Ke and Ka represent the elimination rate constant and absorption rate constant, respectively. All parameter estimates were computed by PKAnalyst. Bioavailability of r-hFSH and the hFSH analogues were estimated from the ratio of AUC (SC)/AUC (IV), at a constant dose (10 IU/kg).

[0121] In Vivo FSH Bioactivity

[0122] Ganirelix Acetate (250 μg) was administered by SC injection for 10 consecutive days to two normally cycling Rhesus monkeys beginning menstrual cycle day 4. The hFSH analogue was administered as a single subcutaneous dose (10 IU/kg) on cycle day 6. Venipuncture was performed daily and serum assayed for estradiol levels from cycle day 2 through cycle day 14. Serum estradiol was measured using an automated Immulite assay (Diagnostic Products Corporation, Los Angeles, Calif.).

[0123] Alternatively, hypophysectomized mice (surgery at 19 days) were purchased from the Charles River Company (Wilmington, Mass.). Upon arrival, mice were rehydrated with glucose-supplemented water for four days and randomized into control and experimental groups. Control recombinant hFSH protein or hFSH analogue was administered via a single subcutaneous injection in a total volume of 100 microliters at a dose of 10 IU. On day four post-injection, the animals were weighed and sacrificed by carbon dioxide asphyxiation followed by cardiopuncture and drainage. The uterus and ovaries were weighed and sectioned for histologic analysis.

[0124] Carbohydrate Analysis

[0125] MALDI-TOF mass spectrometry combined with exoglycosidase digestion was used to determine the carbohydrate composition of the synthetic analogues described herein, using an adaptation of the technique described in Kim et al, 2001. Briefly, disulfide bond reduction is followed by a tryptic digest and/or other enzymatic digests to liberate peptides containing single carbohydrate side chains. The mass of each glycosylation variant is determined by calculating the difference in mass between the observed peptide and its nonglycosylated primary sequence. Further analysis may include N-linked profiling and sequencing, O-linked profiling and monosaccharide composition analysis using “FACE”™ technology kits from (Glyko, Novato, Calif.).

[0126] Ambiguities from the FACE™ analysis were resolved by a compositional analysis. Acid hydrolysis was followed by HPLC chromatography with a CARBOPAC™ PA1 anion exchange column (Dionex, Sunnyvale, Calif.). The carbohydrate content was quantitated by comparing peak areas to a standard mixture. For sialic acid analysis the conditions were appropriately modified. Additionally, techniques such as protein sequencing were used to determine if specific sites were glycosylated in synthetic sequences described herein. Both O-linked and N-linked glycosylated residues are “hidden” from this analysis due to the added carbohydrate. Therefore the “absence” of a serine residue, from a known sequence suggests the presence of a carbohydrate moiety at that position.

[0127] In Vitro FSH Bioactivity

[0128] Bioactivity of the hFSH analogues was evaluated using Y-1 cells transfected with the FSH receptor. Y-1 cell cultures were mixed with the fusion protein and native pituitary hFSH (control) at varying concentrations and media was assayed for cAMP activity as described in Bouloux et al, 2001.

[0129] In Vitro VEGF Bioactivity

[0130] Endothelial Cell Proliferation (metabolism): Human microvascular endothelial cells were used in a standard MTT assay (HMVEC-d Neo, Clonetics, San Diego, Calif.). Briefly, cells were seeded at 3000 cells per well in a 96 well plate in basal medium (EBM, Clonetics) containing 2% FBS, but devoid of growth factors. After a 2-day starvation period, the media was replaced with media containing either the fusion protein or native recombinant VEGF. Two days later this media was replaced with fresh media containing the same factors. Following another three days of culture, the number of actively metabolizing cells was determined using an MTT assay (R & D systems, Minneapolis, Minn.).

[0131] VEGF Receptor Activation: Microvascular endothelial cells isolated from human foreskin were plated at 100,000 cells per well in 12 well plates and cultured for 5 hr in EBM media containing growth factors and other nutrients. The cells were then starved overnight in EBM medium containing 0.2% BSA, with no other additives. Following starvation, approximately 0.5 pmol of either VEGF, the VEGF-FSH fusion protein or hFSH-CTP was added to the medium. After a 1 hr incubation at 4° C., the cells were washed twice in cold PBS containing calcium and magnesium then lysed in a standard RIPA buffer containing protease inhibitors (50 mM HEPES pH 7.2, 10 mM EDTA, 0.1%SDS, 1% NP-40, 0.5% deoxycholate, 50 mM Na-pyrophospate, 100 mM NaF, 2 mM orthovanadate pH 7.5, 1 mM zinc acetate and one protease inhibitor pill per 50 ml of solution (Roche, Indianapolis, Ind.)). The lysate was filtered by centrifugation in a 0.65 micron Durapor PVDF filter (Millipore, Bedford, Mass.) at 3,000 rpm for 30 min. The filtrate was incubated with 10 ug/ml anti-KDR antibody (monoclonal antibody 2-10-1) for 2 hr. Protein A-Sepharose (Amersham/Pharmacia Biotech, Piscataway, N.J.) was added and the mixture was incubated overnight at 4° C. with gentle mixing. The Sepharose was washed twice with RIPA buffer and then boiled for 5 min. in 25 ul 2×SDS-PAGE sample buffer. Supernatants were electrophoresed on a gradient gel of 4-15% acrylamide (BioRad, Hercules, Calif.) and electroblotted onto a PVDF membrane. After blocking, the membrane was washed 3×in PBS with 0.05% Tween 20 (PBS-T). The membrane was incubated overnight in horseradish peroxidase-conjugated anti-phosphotyrosine (10 ng/ml) monoclonal antibody (clone 4G10, Upstate Biotechnology, Lake Placid, N.Y.) in PBS-T containing 3% BSA. The membrane was washed 3×in PBS-T and developed using a chemiluminescent system (ECL, Arnersham Pharmacia Biotech, Piscataway, N.J.).

[0132] In Vivo Bioactivitty

[0133] Hypophysectomized female mice (surgery at 19 days) were purchased from the Charles River Company (Wilmington, Mass.). Upon arrival, mice were rehydrated with glucose-supplemented water for 4 days, and subsequently randomized to one of the following groups (n=5 for each group) : (1) saline, (2) hFSH-CTP, (3) VEGF-FSH, or (4) co-administration of hFSH-CTP+dimeric VEGF. Hormone or control was administered by a single SC injection in a total volume of 100 uL at a dose of 10 IU (mice in group 4 received 10 IU of both hFSH analogue and dimeric VEGF). Mice were housed separately according to group, fed standard mouse feed, and given water supplemented with glucose throughout the study period. On day 4 post-injection, animals were weighed and sacrificed by carbon dioxide asphyxiation followed by cardiopuncture and drainage. The uterus and ovaries of each animal were extirpated, weighed, sectioned and stained with H & E.

[0134] Histologic Preparation and Follicle Counts

[0135] Both ovaries were removed from each animal. One ovary was weighed, immersed in formalin for fixation and embedded in paraffin according to standard protocols. Sections were cut at four to five micron intervals and every tenth section was stained with hematoxylin and eosin. Follicle density and maturation were assessed using the method of Pedersen and Peters (1968).

[0136] Perifollicular capillary density was assessed by counting the number of capillaries under 600×magnification in H & E stained sections, using an ocular containing a grid covering 25 um². Serial sections were counted, with the grid directed over the periphery of follicles (avoiding the avascular center of the follicle).

[0137] Immunostaining for PECAM and Blood Vessel Density Analysis

[0138] The contralateral ovary was embedded in OCT (Tissue-Tek, Torrance, Calif.), snap frozen in isopentane, and stored at −80° C. Frozen ovaries were sectioned at 5 micron intervals. Sections were dehydrated and permiablilzed in 24° C. acetone for 2 min and then rehydrated in phosphate-buffered saline (PBS) containing 1% hydrogen peroxide for 30 min followed by blocking with rabbit serum diluted 1:50 in PBS containing 3% BSA. A rat anti-PECAM antibody was used at a 1:1000 dilution (Vecchi et al, 1994), PharMingen, San Diego, Calif., followed by a biotinylated rabbit anti-rat IgG (Vector Laboratories, Burlingame, Calif.) used at a dilution of 1:2000 for 30 min at room temperature. Visualization of bound antibodies was achieved by incubation with avidin and horseradish-peroxidase-conjugated biotin in PBS (1:100 dilution) for 30 min (Vectastain Standard ABC Elite kit, Vector Laboratories). Peroxidase staining was performed with diaminobenzidine tetrahydrochloride (DAB) as achromogen (DAB Substrate kit, Vector Laboratories, Burlingame, Calif.). Tissues were counterstained with hematoxylin.

[0139] Blood Vessel Density: To determine blood vessel density, PECAM immunostained sections were examined using a 10×10 eyepiece grid under a 40×objective as previously described by Ferrara et al, (1992; 1998). Using this magnification, a grid covers a region of 0.063 mm². Random regions of the follicle perimeter were selected to fill the grid, avoiding the avascular interior of the follicles. Each square within a grid containing a PECAM-positive endothelial cell was counted as one “hit”. Vascular density is expressed as the absolute number of “hits”. An average number of hits from 5 independent, randomly selected ovarian sections from control and treatment animals was routinely used in these analyses.

[0140] Non-ovarian Tissue: Systemic effects of VEGF-FSH were evaluated using immunhistochemical detection of PECAM in snap frozen liver and kidney sections.

[0141] Inhibin Assay

[0142] Inhibin A, produced by developing follicles, provides an indirect measure of follicle number and maturity. Serum inhibin levels from mice and rats injected with the synthetic FSH analogues and control peptides or saline were determined using an enzyme-linked immunoassay specific for the inhibin A dimer (Oxford Bio-Innovation LTD, Oxfordshire, UK). This assay is able to detect rat and mouse inhibin A reproducibly.

RESULTS Example 1

[0143] The β-hFSH-CTP-α Fusion Protein

[0144] In Vitro Bioactivity

[0145] The bioactivity of the β-hFSH-CTP-α analogue was first assessed by an assay of hFSH receptor activity. In this assay, a recombinant native hFSH receptor is expressed in a suitable host cell and cAMP induction is measured following incubation with hormone (Lindau-Shepard et al, 2001). As shown in FIG. 8, the β-hFSH-CTP-α analogue induced a similar rise in cAMP levels when compared with recombinant hFSH, demonstrating that this single-chain fusion analogue folded properly into an unhindered, biologically active hormone.

[0146] Pharmacokinetics

[0147] In order to establish the pharmacokinetic parameters of the instant synthetic FSH, Rhesus monkeys were injected with an IV bolus dose (10 IU/kg) of either a recombinant native hFSH, or the β-hFSH-CTP-α analogue. The serum concentration of hFSH was determined by immunoassay at times following injection and a serum concentration-time curve was generated based on the data. For both the recombinant native hFSH and the β-hFSH-CTP-α analogue, the resulting curve fit a two-compartment model, consisting of an initial distribution half-life and a second, slower, elimination half-life. As indicated by the pharmacokinetic parameter estimates listed in Table 1, the half-life of elimination for the β-hFSH-CTP-α analogue was more than four-fold longer than that of the native hFSH. TABLE 1 Pharmacokinetic parameter estimates after IV bolus injection of rhFSH-CTP or the control recombinant hFSH protein (rhFSH), each at a dose of 10 IU/kg. PARAMETER rhFSH-CTP rhFSH T_(1/2 distribution) (hr) 3.16 1.39 T_(1/2 elimination) (hr) 35.29 8.25 AUC (mIU/ml d) 278 38.8 Clearance (1/kg hr) 1.5 10.74

[0148] Although these results obtained following an intravenous bolus injection were encouraging, it was also important to determine the pharmacokinetic parameters of the synthetic FSH following a subcutaneous injection. This is because subcutaneous administration is a relatively easier route for clinical use. As indicated by the serum concentration-time curves for treatment animals (n=4) receiving the β-hFSH-CTP-α analogue (FIG. 9) and controls (n=2) receiving native hFSH (FIG. 10), the serum levels of native hFSH approached baseline by day 4 post-injection, whereas elevated (>2 mIU/mL) levels of the β-hFSH-CTP-α analogue were maintained for approximately 10 days. These data fit a one-compartment pharmacokinetic model, the parameter estimates of which are given in Table 2. TABLE 2 Mean pharmacokinetic parameter estimates after subcutaneous injection of β-rhFSH-CTP (n = 4) or the control, rhFSH (n = 2), each at a dose of 10 IU/kg. Bioavailability was calculated as AUCsc/AUCiv. PARAMETER rhFSH-CTP rhFSH T_(1/2 elimination) (hr) 35.23 15.74 T_(1/2 absorption) (hr) 5.04 1.75 C_(max) (mIU/ml) 101.26 25.77 T_(max) (hr) 16.39 5.95 AUC (mIU/ml d) 275.31 30.96 Bioavailability (%) 99 80

[0149] Notably, the half-life of absorption for the instant synthetic FSH was approximately threefold longer than that of the native hFSH. These results show that the half-life of elimination correlates well with the intravenous data and confirms the slower metabolism and clearance of the β-hFSH-CTP-α analogue. Addition of the CTP moiety to hFSH thus induced a depot effect, retarding the absorption of the product following subcutaneous administration. This explains the slower time to reach peak concentration (t_(max)) for animals receiving the β-hFSH-CTP-α analogue. As indicated in FIG. 13, both drugs were highly bioavailable after subcutaneous administration.

[0150] In Vivo Bioactivitty

[0151] To establish the bioactivity of the β-hFSH-CTP-α analogue in vivo, two normally cycling monkeys were injected with a single dose of the analogue, and serum was assayed for estradiol at various times following injection. Both monkeys were given a GnRH antagonist (Antagon, Organon, West Orange, N.J.) for the duration of the study, eliminating any effect on ovarian estrogen production from endogenous Rhesus FSH. As shown in FIG. 11, serum estradiol levels initially increased in both animals, with peak levels achieved at 3 and 5 days post-injection. One monkey attained supraphysiologic levels of estradiol (peak 773 pg/mL) on day 4 post-injection, suggesting early recruitment of multiple follicles. Thus, the β-hFSH-CTP-α analogue demonstrated similar, and in one case substantially increased, in vivo biological activity compared to native hFSH.

[0152] Conclusions

[0153] The results presented herein demonstrate that the addition of CTP to the carboxy terminus of the β subunit of hFSH had no adverse impact on folding of the molecule, receptor binding, or in vitro signal transduction. Furthermore, the fusion protein was metabolized at a slower rate than the native hormone, as circulating levels remained elevated for an extended period of time compared to native recombinant hFSH. Quantitatively, the half-life of elimination for the β-hFSH-CTP-α analogue following subcutaneous administration was 2 to 3 times longer than that of native recombinant hFSH. This difference corresponds well with the only previous report on pharmacokinetics in humans, which was done with male subjects, in which the half-life of elimination after subcutaneous administration was prolonged by a similar magnitude compared with historic controls receiving native hormone.

[0154] These results also confirm the accuracy of our parameter estimate for elimination half-life by assessing pharmacokinetics after IV administration. Surprisingly, absorption of the β-hFSH-CTP-α analogue was delayed by approximately three-fold following subcutaneous administration. The long circulating presence of the β-hFSH-CTP-α analogue after subcutaneous administration is thus explained not only by a decreased metabolism of the protein, but by a depot effect resulting in slower absorption.

[0155] In summary, the pharmacodynamics and biological activity of a β-hFSH-CTP-α analogue in a primate model are described herein for the first time. Administration of the β-hFSH-CTP-α analogue to 2 monkeys given a GnRH antagonist (to suppress endogenous FSH activity) elicited a dramatic rise in serum estradiol levels. A single subcutaneous dose resulted in elevated estradiol levels for 5-7 days, with one monkey achieving a peak estradiol level greater than 3 times that seen during a normal endogenous Rhesus cycle.

[0156] This supraphysiologic response is indicative of multifollicular recruitment, although sonographic confirmation was not performed. Such prolonged elevations in estradiol are not normally seen after isolated subcutaneous injections of native recombinant hFSH.

[0157] These results confirm the feasibility of achieving prolonged ovarian stimulation following a single injection of a recombinant gonadotropin analogue. Fewer injections will result in less patient discomfort, improved compliance, and possibly a reduction in the number of local side effects.

[0158] Combination therapy using both long and short-acting FSH formulations, either together and/or sequentially during a stimulation cycle, should also be considered. In these cases, the short-acting (native) formulation may be used to “fine-tune” the FSH dose after an initial bolus of a long-acting analog.

[0159] Ideal candidates for treatment with long-acting FSH analogues include infertile males with hypogonadotropic hypogonadism, who typically require prolonged courses of gonadotropin therapy. This technology also provides a significant improvement over current methods for stimulating follicular maturation and egg production in a subject being treated for infertility and for in vitro fertilization protocols.

Example 2

[0160] The β-hFSH-N2/N4-α Fusion Protein

[0161] In Vitro Bioactivity

[0162] The bioactivity of the β-hFSH-N2/N4-α analogues was first assessed by an assay for hFSH receptor activation as discussed above. The N2/N4 analogue induced a similar rise in cAMP levels when compared with native hFSH, demonstrating that, like the CTP analogue discussed above, this single-chain fusion protein folded properly into an unhindered, biologically active hormone.

[0163] Pharmacokinetics

[0164] Pharmacokinetic analysis was performed using twelve immature female rats divided into four groups of 3 each. Each of the three proteins (hFSH, hFSH-CTP, hFSH-N2) was diluted to 11 μg/ml in injection buffer containing BSA (1 mg/ml), and given as a single intravenous dose of 2800 ng/rat in 0.25 ml of buffer. The control group received 0.25 ml of saline (data not shown). Serum was assayed at the following intervals post-injection: 0.5, 1.0, 3.0, 6.0, and 12 hours. The serum concentration-time curves are shown in FIG. 18. For all products the curves could be explained by a two-compartment model, with an initial half-life reflecting the distribution phase, and a second, slower elimination half-life. As indicated by the pharmacokinetic parameter estimates in Table 3, the half-life of elimination for the synthetic FSHs, hFSH-CTP and hFSH-N2, was approximately two-fold longer than that of native hFSH (3.5 h vs 7.1 and 6.3 h, respectively). TABLE 3 Mean pharmacokinetic parameter estimates after IV bolus injection of rhFSH, rhFSH-CTP or hFSH-N2, each at a dose of 2800 ng/rat (n = 3). AUC = area under the curve. PARAMETER rhFSH rhFSH-CTP rhFSH-N2 AUC_(0-infinity) (ng/hr/ml) 1491 3887 4802 T_(1/2 elimination (hr)) 3.7 7.1 7.3 Clearance (ml/hr) 1.9 0.72 0.58

[0165] In Vivo Bioactivity

[0166] An ovarian weight gain assay was used to assess the relative in vivo bioactivity of the control, recombinant native FSH (rFSH) and the CTP and N2 FSH analogues. The compounds were administered in a single subcutaneous injection. The mean ovarian weights as determined on day three following injection are shown in FIG. 17.

[0167] Pharmacodynamics

[0168] The pharmacodynamics of the N2/N4 analogues were assessed by a determination of ovarian weight change in immature female rats following a single subcutaneous injection of either a recombinant native hFSH, the CTP, the N2, or the N4 analogue. The results of these analyses are summarized in FIG. 19. The data indicated that the mean ovarian weights three days post-injection were significantly higher for the CTP, N2 and N4 analogues compared with the native hFSH control or saline.

[0169] Conclusions

[0170] The results described herein demonstrate that the addition of N-linked carbohydrates imparts a longer half-life to native hFSH, thereby increasing its bioactivity in a manner analogous to that conferred by the O-linked sugars on the CTP.

[0171] These results further demonstrate that a synthetic sequence bearing artificial N-linked glycosylation consensus sequences can be efficiently glycosylated in cultured cells. This in turn demonstrates the feasibility of producing synthetic FSH having improved stability and bioactivity through directed modifications of glycosylation patterns via the addition of artificial sequences.

Example 3

[0172] The VEGF-β-hFSH-CTP-α Fusion Protein

[0173] In Vitro Bioactivity

[0174] Purified VEGF-FSH was tested for both FSH and VEGF activity. In vitro FSH activity was examined using a cell line expressing the hFSH receptor as described in the methods section. As shown in FIG. 19, the VEGF-FSH fusion protein exhibited similar in vitro bioactivity compared to recombinant hFSH.

[0175] The biological activity of the VEGF portion of the chimera, was confirmed in a number of in vitro assays. First, the ability of VEGF-FSH to stimulate the proliferation of endothelial cells was evaluated using an MTT assay. The MTT assay measures metabolic activity which is used here as an indicator of cell proliferation. The ability of the synthetic VEGF-FSH to proliferate human micro vascular endothelial cells is demonstrated in FIG. 20A. Notably, metabolism as determined with the MTT assay was directly proportional to the concentration of the VEGF-FSH fusion protein in the media. The response observed was similar to that induced by the same concentrations of commercially available preparations of VEGF (Becton Dickinson Labware, Franklin Lakes, N.J.).

[0176] The supernatant of CHO cells transfected with the VEGF-FSH fusion protein was also examined for its effects on cell metabolism in the MTT assay. As shown in FIG. 20B, supernatant from VEGF-FSH-secreting cells induced a response comparable to that of complete medium which is rich in numerous growth factors (EBM plus VEGF, hFGF-β, hEGF and R3-IGF-1; growth factors supplied by Clonetics and added in concentrations specified by the manufacturer). In contrast, the supernatant of cells secreting synthetic FSH-CTP alone induced only a modest response, indicating that the VEGF component is active and contributing significantly to the response observed with-the VEGF-FSH fusion protein.

[0177] The primary molecular response elicited by a growth factor is the autophosphorylation of its cognate receptor, which is induced upon ligand binding. Therefore, the ability of VEGF-FSH to induce phosphorylation of the VEGF receptor was tested. Western blotting and immunodetection with phosphotyrosine-specific antibodies was used to detect the phosphorylation state of the receptor. As illustrated in FIG. 21, cells treated with VEGF-FSH induced a level of receptor phosphorylation similar to that induced by native VEGF. The absence of receptor phosphorylation in cells treated with hFSH-CTP indicates that the observed phosphorylation is due to of the VEGF portion of our chimeric molecule.

[0178] In Vivo Bioactivity

[0179] The in viva bioactivity of the VEGF-FSH fusion protein was evaluated by examining its effects on follicular development in mice. Immature, hypophysectomized female mice were used in these studies because they lack appreciable endogenous FSH. The ovaries of hypophysectomized female mice were harvested for analysis four days following a single subcutaneous injection of either saline, hFSH-CTP, VEGF-FSH, or both hFSH-CTP and VEGF administered together. As shown in FIG. 22, induction of follicular maturation was evident in all groups except that receiving saline. The presence of stage 6-7, preantral to early antral, follicles is indicated in the figure by closed arrows. In contrast, animals from the saline control group 1 did not show progression beyond a stage 5 follicle, as indicated by multiple granulosa cell layers, but no early antrum formation. This is consistent with complete gonadotropin deprivation in this group.

[0180] Notably, stromal vessels taken from animals injected with VEGF-FSH were engorged compared with those from animals receiving either hFSH-CTP or the combination of VEGF and hFSH-CTP (FIG. 23, open arrows). Perifollicular capillary density was assessed as described in the methods section. A representative H&E section is shown in FIG. 23. The number of capillaries per square millimeter was 50 for saline, 300 for hFSH-CTP, 380 for VEGF-FSH and 300 for the combination of both hFSH-CTP and VEGF. These results indicate that the VEGF-FSH fusion protein achieved enhanced VEGF activity at the ovary compared to synthetic FSH alone and FSH-CTP plus free VEGF. Furthermore, these results indicate that the VEGF moiety of the VEGF-FSH fusion has an extended in vivo half-life compared to free VEGF.

[0181] Targeting of the VEGF-FSH-CTP

[0182] To test whether the VEGF activity of the single-chain VEGF-FSH-CTP chimera was systemic or whether VEGF activity was targeted to the ovary, adult mice were given a single injection of either: (1) saline (2) VEGF-FSH-CTP, (3) commercially available VEGF, or (4) VEGF-N2. No serious illness was noted in animals receiving commercially available VEGF, most likely due to its short half-life in vivo. In contrast, the animals receiving VEGF-N2 exhibited signs of lethargy, tachypnea, tachycardia, and generalized edema consistent with prolonged systemic VEGF activity. Surprisingly, animals receiving VEGF-FSH-CTP exhibited no gross adverse effects. Animals were sacrificed at 12 hours and 24 hours post-injection and the liver and pituitary were collected, sectioned, and evaluated via H&E staining for evidence of deleterious VEGF activity, such as micro- vascular bleeding. The results confirmed that the VEGF- FSH-CTP glycoprotein exhibited negligible extrα-ovarian VEGF affects, even though it had a substantially increased half-life relative to native VEGF. Thus, the incorporation of the FSH moiety successfully promoted the localization of the VEGF-FSH-CTP glycoprotein at the target tissue.

[0183] Discussion

[0184] The data disclosed herein demonstrate the feasibility of producing multifunctional hormone analogues to achieve both improved in vivo stability and activity at the site of the target tissue. The single chain chimeric construct provided herein is a relatively large multidomain protein. These are the first demonstration that such a protein can be produced in a manner that retains the biological activity of each independent factor.

[0185] Multi-functional hFSH constructs are useful for targeting a bioactive protein to the ovary. This technique may be also be adapted to target factors to other tissues by replacing hFSH with other tissue-specific factors.

[0186] The results herein also demonstrate that a synthetic VEGF-hFSH-CTP protein can induce neovascularization as well as increase delivery of blood through existing ovarian vasculature. This will improve follicular recruitment and growth, processes known to be dependent on neovascuoarization and mediated, at least in part, by endogenous VEGF.

[0187] The methods described herein can also be used to tether other growth factors to hFSH, for example, to treat infertility. Particularly promising factors include TGFα and β1, EGF (epidermal growth factor), SCF (stem cell factor), IGF (insulin-like growth factor) I & II, and KGF (keratinocyte growth factor). For each of these factors the mature polypeptide that encodes the minimal domain necessary to exert an effect can be used for fusion with the hFSH-CTP construct.

[0188] The modularity of this approach allows different orientations to be used to achieve maximal activity. For growth factors such as EGF or TGF-β1, wherein the mature peptide is buried within a much larger pro-protein, the signal sequence of the β-hFSH subunit can be used to drive secretion.

[0189] For construction of other growth factor FSH fusion proteins, clones may either be acquired from the-American Type Culture Collection or the appropriate cDNAs may be isolated by RT-PCR from cell lines or tissues using standard techniques. Select domains may be synthesized for expression when the active polypeptide is small (e.g., EGF).

[0190] For example, an EGF-FSH fusion can be produced as follows. The small EGF cDNA encoding the 53 amino acids of the mature protein is isolated and ligated in-frame at the carboxy terminus of the hFSH-CTP clone. The hFSH α-subunit terminator codon is deleted while another terminator codon is added at the C-terminal end of EGF. An amino-terminal fusion is generated by placing the cDNA encoding the 53 residues of EGF between the signal peptide of the β-hFSH subunit and the first residue of the mature β-hFSH (while maintaining residues required for cleavage of the signal peptide). This sequence utilizes the signal peptide of β-hFSH to drive secretion in the absence of the signal sequence from EGF at the amino terminus of the fusion protein.

[0191] TGF α and β1 fusion proteins may be produced using a methodology analogous to that used for EGF. For example, the cDNA encoding the 50 residues of the mature TGFα and that encoding the 112 residues of the mature TGFβ1 can be fused to the hFSH-CTP molecule at the amino and carboxy terminal ends. Again, the signal sequence of the β-hFSH moiety is used to drive secretion of the amino terminal fusions by placing the growth factors between the β-hFSH signal sequence and the first residue of the mature β-hFSH polypeptide.

[0192] By way of further example, an IGF-FSH-CTP fusion can be made by ligating the 70 and 67 residue active polypeptides of IGF I and II, respectively, to FSH-CTP by either of two methods. In the first, a cDNA encoding the active domains is inserted into the FSH-CTP clone as described for EGF and TGFα and β1. Alternatively, IGF can be tethered to the FSH-CTP at the amino terminus using its native signal sequences and removing the signal sequence form the β-hFSH moiety as was described for the VEGF-FSH construct.

[0193] An SCF-hFSH-CTP fusion can be made as described for IGF I and II. However, this SCF possesses both soluble and membrane associated domains. The transmembrane and intracellular domains are at the carboxy terminus of the protein. Therefore the expressed protein is truncated to consist of the 165 residues comprising the mature, active, soluble peptide. Thus, the SCF protion of the fusion construct terminates at the sequence: ASSLR. Alternatively, the native signal sequence of SCF may be used for secretion if an additional 25 residues are included at the amino terminus of the polypeptide.

[0194] A final example is the KGF-FSH-CTP fusion, in which a cDNA encompassing the entire coding domain (582 bp) is fused to hFSH-CTP at the amino terminus utilizing the signal sequence of KGF to drive secretion of the fusion protein. This linkage sequence removes the signal sequence from hFSH-CTP as was described for the VEGF-FSH fusion. Alternatively, a carboxy-terminal fusion of the 163 residue mature KGF protein can be made. The terminator codon of the C-terminal α-subunit is removed and placed instead after the KGF domain.

[0195] All of the above fusions include a Ser-Gly tether between the growth factor and the hFSH-CTP. This flexible tether alleviates any inhibitory steric effects resulting from the close proximity of the active domains of these multidomain fusion proteins.

[0196] Naturally, the production of tissue-directed molecular therapy has applications beyond infertility. Localization of VEGF to the heart after cardiac infarction or to skin for improved would-healing are also potential candidates for this type of therapy. Numerous other therapies to target other growth factors to specific tissues of interest may also be worthwhile. Our therapeutic design avoids many of the complications of gene therapy, like exposure to viral constructs to carry the gene of interest, difficulty with tissue specificity, and inefficiency of genomic integration. In summary, we have shown that through the use of hyper-glycosylation to improve half-life and careful protein design, the creation of biologically active, tissue-specific fusion proteins is possible.

REFERENCES

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What is claimed is:
 1. A composition of matter comprising (a) at least one subunit of a hormone or growth factor and (b) a half-life-increasing moiety, wherein the hormone or growth factor subunit and half-life-increasing moiety are covalently bound.
 2. The composition of claim 1, wherein the hormone or growth factor is selected from the group consisting of VEGF, GH, TGFα, TGFβ1, EGF, FGF, SCF, IGF-I, IGF-II, GDF-9, KGF, BMP-15, GM-CSF, LIF, follistatin, activin-β, a neurotropin, cadherin, angiopoiten I or II, MCP-1, ICAM-1, CD18, P-selectin and MIP-2.
 3. The composition of claim 2, wherein the growth factor is VEGF.
 4. The composition of claim 1, wherein the hormone or growth factor is from an animal selected from the group consisting of a primate, a horse, a sheep, a bird, a bovine, a pig, a dog, a cat, and a rodent.
 5. The composition of claim 4, wherein the hormone or growth factor subunit is from a human.
 6. The composition claim 1, wherein the half-life-increasing moiety is a non-peptidyl polymer.
 7. The composition claim 1, wherein the half-life-increasing moiety is a polypeptide segment.
 8. The composition of claim 7, wherein the hormone or growth factor subunit and the polypeptide segment exist within a single polypeptide chain.
 9. The composition of claim 8, wherein the hormone or growth factor subunit is bound at its C-terminal end to the N-terminal end of the polypeptide segment.
 10. The composition of claim 8, wherein the hormone or growth factor subunit is bound at its N-terminal end to the C-terminal end of the polypeptide segment.
 11. The composition of claim 7, wherein the polypeptide segment comprises the carboxy-teminal portion of the β-hCG subunit.
 12. The composition of claim 11, wherein the carboxy-terminal portion of the β-hCG subunit comprises the amino acid sequence corresponding to positions 113-145 of the β-hCG subunit.
 13. The composition of claim 12, wherein the carboxy-terminal portion of the β-hCG subunit is glycosylated.
 14. The composition of claim 13, wherein the polypeptide segment comprises an O-linked glycosylation site.
 15. The composition of claim 13, wherein the polypeptide segment further comprises a region having an N-linked glycosylation site.
 16. The composition of claim 15, wherein the number of glycosylation sites is one, two, three, four, five or six sites.
 17. The composition of claim 15, wherein the polypeptide segment contains a plurality of glycosylation sites, each site being separated from its adjacent site by about six amino acid residues.
 18. The composition of claim 7, wherein the polypeptide segment comprises the amino acid sequence ser-gly-ser-asn-ala-thr-gly-ser-gly-ser-asn-ala-thr-ser-gly-ser.
 19. The synthetic molecule of claim 7, wherein the polypeptide segment comprises a plurality of the amino acid sequence ser-gly-ser-asn-ala-thr-gly-ser-gly-ser-asn-ala-thr-ser-gly-ser.
 20. A composition of matter comprising (a) at least one subunit of a hormone or growth factor, (b) a half-life-increasing moiety, and (c) a moiety that specifically binds to cells of a predetermined tissue.
 21. A composition of matter comprising (a) at least one subunit of a hormone or growth factor, (b) a β-FSH subunit, (c) an α-FSH subunit and (d) a half-life-increasing moiety, wherein the hormone or growth factor, the β-FSH subunit, the α-FSH subunit and the half-life-increasing moiety are covalently bound.
 22. The composition of claim 21, wherein the hormone or growth factor is selected from the group consisting of VEGF, GH, TGFα, TGFPβ1, EGF, FGF, SCF, IGF-I, IGF-II, GDF-9, KGF, BMP-15, GM-CSF, LIF, follistatin, activin-β, a neurotropin, cadherin, angiopoiten I or II, MCP-1, ICAM-1, CD18, P-selectin and MIP-2.
 23. The composition of claim 22, wherein the growth factor is VEGF.
 24. The composition of claim 21, wherein the hormone or growth factor is from an animal selected from the group consisting of a primate, a horse, a sheep, a bird, a bovine, a pig, a dog, a cat and a rodent.
 25. The composition of claim 24, wherein the hormone or growth factor is from a human.
 26. The composition of claim 23, wherein the α-FSH and β-FSH subunits are from an animal selected from the group consisting of a primate, a horse, a sheep, a bird, a bovine, a pig, a dog, a cat and a rodent.
 27. The composition of claim 26, wherein the α-FSH and β-FSH subunits are human α-FSH and β-FSH subunits.
 28. The composition of claim 23, wherein the β-FSH subunit and α-FSH subunit are bound to each other via the half-life-increasing moiety.
 29. The composition of claim 23, wherein the half-life-increasing moiety is a non-peptidyl polymer.
 30. The composition of claim 23, wherein the half-life-increasing moiety is a polypeptide segment.
 31. The composition of claim 30, wherein the VEGF, the β-FSH subunit, the α-FSH subunit and the polypeptide segment exist within a single polypeptide chain.
 32. The composition of claim 31, wherein the β-FSH subunit is bound at its C-terminal end to the N-terminal end of the polypeptide segment, and the polypeptide segment is bound at its C-terminal end to the N-terminal end of the α-FSH subunit.
 33. The composition of claim 32, wherein the synthetic FSH comprises the N-terminal signal sequence of the β-FSH subunit.
 34. The composition of claim 31, wherein the α-FSH subunit is bound at its C-terminal end to the N-terminal end of the polypeptide segment, and the polypeptide segment is bound at its C-terminal end to the N-terminal end of the β-FSH subunit.
 35. The composition of claim 31, wherein the VEGF is bound at its C-terminal end to the β-FSH subunit, the β-FSH is bound at its C-terminal end to the N-terminal end of the polypeptide segment, and the C-terminal end of the polypeptide segment is bound to the N-terminal end of the α-FSH subunit.
 36. The composition of claim 30, wherein the polypeptide segment comprises the carboxy-terminal portion of the β-hCG subunit.
 37. The composition of claim 36, wherein the carboxy-terminal portion of the β-hCG subunit comprises the amino acid sequence corresponding to positions 113-145 of the β-hCG subunit.
 38. The composition of claim 37, wherein the carboxy-terminal portion of the β-hCG subunit is glycosylated.
 39. The composition of claim 38, wherein the polypeptide segment comprises an O-linked glycosylation site.
 40. The composition of claim 38, wherein the polypeptide segment further comprises a region having an N-linked glycosylation site.
 41. The composition of claim 40, wherein the number of glycosylation sites is one, two, three, four, five or six sites.
 42. The composition of claim 40, wherein the polypeptide segment contains a plurality of glycosylation sites, each site being separated from its adjacent site by about six amino acid residues.
 43. The composition of claim 30, wherein the polypeptide segment comprises the amino acid sequence ser-gly-ser-asn-ala-thr-gly-ser-gly-ser-asn-ala-thr-ser-gly-ser.
 44. The composition of claim 43, wherein the polypeptide segment contains one copy of the amino acid sequence ser-gly-ser-asn-ala-thr-gly-ser-gly-ser-asn-ala-thr-ser-gly-ser.
 45. The composition of claim 43, wherein the polypeptide segment comprises a plurality of the amino acid sequence ser-gly-ser-asn-ala-thr-gly-ser-gly-ser-asn-ala-thr-ser-gly-ser.
 46. A pharmaceutical composition comprising the composition of claim 1, 3, 20, 21 or 23, and a pharmaceutically acceptable carrier.
 47. A nucleic acid encoding the polypeptide chain of the composition of claim 8 or
 31. 48. A vector that comprises the nucleic acid of claim
 47. 49. The vector of claim 48, wherein the vector is a plasmid, cosmid, λ phage or YAC.
 50. A cell which comprises the vector of claim
 48. 51. The cell of claim 50, wherein the cell is a bacterial cell or a eucaryotic cell.
 52. The cell of claim 51, wherein the cell is E. coli.
 53. A method for producing a polypeptide that comprises growing the cell of claim 50 under conditions permitting expression of the polypeptide encoded by the vector therein, and recovering the polypeptide so expressed.
 54. A method for increasing a subject's fertility which comprises administering to the subject an amount of the composition of claim 23 effective to enhance the subject's fertility.
 55. The method of claim 54, wherein the subject is infertile and desires in vitro fertilization.
 56. The method of claim 55, wherein the subject is a human.
 57. The method of claim 55, wherein the composition is administered daily.
 58. The method of claim 55, wherein the composition is administered every other day.
 59. The method of claim 55, wherein the composition is administered every 6 to 8 days.
 60. The method of claim 55, wherein the composition is administered weekly.
 61. The method of claim 55, wherein the composition is administered intravenously, subcutaneously, intramuscularly, intraperitoneally, orally or topically.
 62. A method for increasing a subject's spermatogenesis which comprises administering to the subject an amount of the composition of claim 23 effective to enhance the subject's spermatogenesis.
 63. A method for increasing a subject's egg production which comprises administering to the subject an amount of the composition of claim 23 effective to enhance the subject's egg production.
 64. The method of claim 55, 62 or 63, wherein the subject is selected from the group consisting of a primate, a horse, a sheep, a bird, a bovine, a pig, a dog, a cat and a rodent.
 65. A method for increasing vascularization in a tissue by contacting the tissue with an amount of the composition of claim 3 effective to increase vascularization in the tissue.
 66. A method for increasing vascularization in ovarian tissue comprising contacting the ovarian tissue with an amount of the composition of claim 23 effective to increase vascularization in the ovarian tissue. 